Spectrophotometer
A spectrophotometer measures the number of photons emitted to estimate the intensity of light spectra absorbed and transmitted by a sample. This provides information on the amount of a compound in the sample.
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| Technical specifications | Description |
| Wavelength selection | Monochromator |
| Light source | Xenon flash lamp |
| Wavelength range | 200–1,000 nm with 1 nm steps |
| Readout range | Up to 4 absorbance units |
| Bandwidth | <2.5 nm |
| Linearity at 450 nm* | 0–2.5 Abs, 2% |
| Accuracy at 450 nm* | 1.0% + 0.003 Abs (0–2.0 Abs), 2.0% (2.0–2.5 Abs) |
| Precision at 450 nm* | SD <0.003 Abs or CV <1.0% |
| Plates | 6- to 48-, 96-, and 6-384-well plates***, Thermo Scientific µDrop Plates |
| Cuvette | Dimensions (H x W x D): 40–58 x 12.5 x 12.5 mm
Beam center height: 8.5 mm Beam window: ≥2 mm |
| Measurement speed
(starting from A1 back to A1) |
6 sec with 96-well plate
10 sec with 384-well plate |
| Plate shaking | Linear |
| Spectral scanning speed | 10 sec from 200–1,000 nm with 1 nm steps |
| Incubation range | From ambient temperature +2C to 45˚C”. |
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